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goat anti otx2  (R&D Systems)


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    R&D Systems goat anti otx2
    (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the <t>OTX2+</t> neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.
    Goat Anti Otx2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti otx2/product/R&D Systems
    Average 96 stars, based on 273 article reviews
    goat anti otx2 - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Wnt pathway modulation is required to correctly execute multiple independent cellular dynamic programs during cranial neural tube closure"

    Article Title: Wnt pathway modulation is required to correctly execute multiple independent cellular dynamic programs during cranial neural tube closure

    Journal: bioRxiv

    doi: 10.1101/2024.12.19.629501

    (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the OTX2+ neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.
    Figure Legend Snippet: (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the OTX2+ neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.

    Techniques Used: Control, Mutagenesis, Staining, Marker



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    (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the <t>OTX2+</t> neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.
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    (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the <t>OTX2+</t> neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.
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    Image Search Results


    (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the OTX2+ neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.

    Journal: bioRxiv

    Article Title: Wnt pathway modulation is required to correctly execute multiple independent cellular dynamic programs during cranial neural tube closure

    doi: 10.1101/2024.12.19.629501

    Figure Lengend Snippet: (A-B) Transverse sections through control and Lrp6 mutant embryos at E7.5, stained with (A) an antibody against OTX2and counterstained with Phalloidin (F-actin) and, or (B) the S-M phase marker phospho-HistoneH3. Anterior is to the left, dashed white lines indicate the OTX2+ neuroepithelial region. (C) Quantification of the percentage of the epiblast tissue marked with OTX2 shows that anterior fates are not expanded in Lrp6 mutants (control: 61.67 ± 6.03 %; Lrp6 : 63.67 ± 3.06 %; n = 3control, 3 Lrp6 embryos at E7.5; p = 0.6352 by unpaired t-test). (D) Lrp6 mutants show a significantly increase in the proportion of proliferative cells in the anterior (OTX2+) neural tissues as compared to controls (control: 5.23 ± 0.97 %; Lpr6 : 9.38 ± 1.25%; n = 3control, 3 Lrp6 embryos; p = 0.0105 by unpaired t-test). (E) No difference is observed in the proportion of proliferative cells between control and Lrp6 mutants in the posterior (OTX2-) neural tissues (control: 5.74 ± 2.08 %; Lrp6 : 6.33 ± 0.95 %; n = 3 control, 3 Lrp6 embryos; p = 0.6783 by unpaired t-test). Scale bars represent 100 µm; A, anterior; P, posterior.

    Article Snippet: Primary antibodies used in this study were: rabbit anti-N-cadherin (1:500, Cell Signaling #13116), mouse anti-phospho-HistoneH3 (1:500, Cell Signaling #9706), rabbit anti-phospho-Myosin Regulatory Light Chain 2 (1:100, Cell Signaling 3674), rat anti-VANGL2 (1:100, Millipore MABN750), and goat anti-OTX2 (1:500, R&D Systems AF1979).

    Techniques: Control, Mutagenesis, Staining, Marker

    Journal: eLife

    Article Title: A multiplexed, single-cell sequencing screen identifies compounds that increase neurogenic reprogramming of murine Muller glia

    doi: 10.7554/eLife.92091

    Figure Lengend Snippet:

    Article Snippet: Goat anti-OTX2 , R&D Systems , Cat#: BAF1979 , RRID: AB_2157171 , 1:500.

    Techniques: Staining

    Journal: Cell reports

    Article Title: Synaptic plasticity in human thalamocortical assembloids

    doi: 10.1016/j.celrep.2024.114503

    Figure Lengend Snippet:

    Article Snippet: Goat polyclonal anti-OTX2 , R&D Systems , Cat# AF1979; RRID:AB_2157172.

    Techniques: Recombinant, Plasmid Preparation, Software